Author:
Sawaswong Vorthon,Chanchaem Prangwalai,Kemthong Taratorn,Warit Saradee,Chaiprasert Angkana,Malaivijitnond Suchinda,Payungporn Sunchai
Abstract
AbstractThe wild-born long-tailed macaques (Macaca fascicularis) were recently recruited and used as breeders for the National Primate Research Center of Thailand, Chulalongkorn University (NPRCT-CU), and changes in their in-depth gut microbiota profiles were investigated. The Oxford Nanopore Technology (ONT) was used to explore full-length 16S rDNA sequences of gut microbiota in animals once captured in their natural habitat and 1-year following translocation and housing in a hygienic environment at NPRCT-CU. Our findings show that the gut microbiota of macaques after 1 year of hygienic housing and programmed diets feeding was altered and reshaped. The prevalent gut bacteria such as Prevotella copri and Faecalibacterium prausnitzii were enriched after translocation, causing the lower alpha diversity. The correlation analysis revealed that Prevotella copri, Phascolarctobacterium succinatutens, and Prevotella stercorea, showed a positive correlation with each other. Significantly enriched pathways in the macaques after translocation included biosynthesis of essential amino acids, fatty acids, polyamine and butanoate. The effects of microbiota change could help macaques to harvest the energy from programmed diets and adapt their gut metabolism. The novel probiotics and microbiota engineering approach could be further developed based on the current findings and should be helpful for captive animal health care management.
Funder
Thailand Government’s Development and Promotion of Science and Technology Talents Project
NIH Graduate Partnerships Program
NSRF via the Program Management Unit for Human Resources and Institutional Development, Research and Innovation
TSRI Research Team Promotion Grant
Thailand Science Research and Innovation Fund, Chulalongkorn University
Ratchadapisek Sompot Fund, Faculty of Medicine, Chulalongkorn University
Publisher
Springer Science and Business Media LLC
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