Author:
Qian Jin,Wu Chun-Yuan,Wu Dong-Ming,Li Li-Hua,Li Qi,Deng Tang,Huang Qi-Feng,Xu Shuang-Qin,Wang Hang-Fei,Wu Xin-Xin,Cheng Zi-Yi,Lv Chuan-Zhu,Liu Xiao-Ran
Abstract
AbstractParaquat (PQ) is a widely used fast-acting pyridine herbicide. Accidental ingestion or self-administration via various routes can cause severe organ damage. Currently, no effective antidote is available commercially, and the mortality rate of poisoned patients is exceptionally high. Here, the efficacy of anthrahydroquinone-2-6-disulfonate (AH2QDS) was observed in treating PQ poisoning by constructing in vivo and ex vivo models. We then explored the detoxification mechanism of AH2QDS. We demonstrated that, in a rat model, the PQ concentration in the PQ + AH2QDS group significantly decreased compared to the PQ only group. Additionally, AH2QDS protected the mitochondria of rats and A549 cells and decreased oxidative stress damage, thus improving animal survival and cell viability. Finally, the differentially expressed genes were analysed in the PQ + AH2QDS group and the PQ group by NextGen sequencing, and we verified that Nrf2’s expression in the PQ + AH2QDS group was significantly higher than that in the PQ group. Our work identified that AH2QDS can detoxify PQ by reducing PQ uptake and protecting mitochondria while enhancing the body's antioxidant activity.
Funder
Hainan Provincial Natural Science Foundation of China
the Youth Cultivation Fund Project of the First Affiliated Hospital of Hainan Medical University
Hainan Province Key R&D Program
National Natural Science Foundation of China
Hainan Province Clinical Medical Center
Publisher
Springer Science and Business Media LLC
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