Abstract
Abstract
Members of the genus Penicillium are commonly isolated from various terrestrial and marine environments, and play an important ecological role as a decomposer. To gain insight into the ecological role of Penicillium in intertidal zones, we investigated the Penicillium diversity and community structure using a culture-dependent technique and a culture independent metagenomic approach using ITS (ITS-NGS) and partial β-tubulin (BenA-NGS) as targets. The obtained isolates were tested for halotolerance, enzyme activity, and polycyclic aromatic hydrocarbons (PAHs) degradation. A total of 96 Penicillium species were identified from the investigated intertidal zones. Although the BenA-NGS method was efficient for detecting Penicillium, some species were only detected using conventional isolation and/or the ITS-NGS method. The Penicillium community displayed a significant degree of variation relative to season (summer and winter) and seaside (western and southern coast). Many Penicillium species isolated in this study exhibited cellulase and protease activity, and/or degradation of PAHs. These findings support the important role of Penicillium in the intertidal zone for nutrient recycling and pollutant degradation.
Funder
Korea Institute of Marine Science and Technology promotion
Publisher
Springer Science and Business Media LLC
Reference94 articles.
1. Pitt, J. I. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces (Academic Press, 1979).
2. Frisvad, J. C. & Samson, R. A. Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Stud. Mycol. 49, 1–174 (2004).
3. Visagie, C. M. et al. Identification and nomenclature of the genus Penicillium. Stud. Mycol. 78, 343–371 (2014).
4. Paz, Z. et al. Diversity and potential antifungal properties of fungi associated with a Mediterranean sponge. Fungal Divers. 42, 17–26 (2010).
5. Park, M. S. et al. Marine-derived Penicillium in Korea: diversity, enzyme activity, and antifungal properties. Antonie van Leeuwenhoek 106, 331–345 (2014).
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