Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle

Author:

Larzábal Mariano,Da Silva Wanderson Marques,Multani Anmol,Vagnoni Lucas E.,Moore Dadin P.,Marin Maia S.,Riviere Nahuel A.,Delgado Fernando O.,Vilte Daniel A.,Victorica Matias Romero,Ma Tao,Le Guan Luo,Talia Paola,Cataldi Angel,Cobo Eduardo R.

Abstract

AbstractThe zoonotic enterohemorrhagicEscherichia coli(EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance ofNegativibacillusandErysipelotrichaceaein mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.

Funder

Fondo para la Investigación Científica y Tecnológica

Natural Sciences and Engineering Research Council of Canada

Alberta Agriculture and Forestry

Eyes High International Collaborative Grant for Young Researchers, University of Calgary

Major Innovation Fund Program for the AMR – One Health Consortium

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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