Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation

Author:

Sakdapetsiri Chatsuda,Kaokhum Nitchakarn,Pinyakong OnruthaiORCID

Abstract

AbstractExiguobacterium sp. AO-11 was immobilized on bio-cord at 109 CFU g−1 carrier for the removal of crude oil from marine environments. To prepare a ready-to-use bioremediation product, the shelf life of the immobilized cells was calculated. Approximately 90% of 0.25% (v/v) crude oil removal was achieved within 9 days when the starved state of immobilized cells was used. The oil removal activity of the immobilized cells was maintained in the presence of oil dispersant (89%) and at pH values of 7–9. Meanwhile, pH, oil concentration and salinity affected the oil removal efficacy. The immobilized cells could be reused for at least 5 cycles. The Arrhenius equation describing the relationship between the rate of reaction and temperature was validated as a useful model of the kinetics of retention of activity by an immobilized biocatalyst. It was estimated that the immobilized cells could be stored in a non-vacuum bag containing phosphate buffer (pH 7.0) at 30 °C for 39 days to retain the cells at 107 CFU g−1 carrier and more than 50% degradation activity. These results indicated the potential of using bio-cord-immobilized crude oil-degrading Exiguobacterium sp. AO-11 as a bioremediation product in a marine environment.

Funder

the Ratchadapisek Somphot Fund for Postdoctoral Fellowship, Chulalongkorn University

the Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Thailand

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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