Author:
Mohamed Hanan R. H.,Farouk Ahmed H.,Elbasiouni Salma H.,Nasif Kirolls A.,Safwat Gehan
Abstract
AbstractCalcium hydroxide (Ca(OH)2NPs), calcium titanate (CaTiO3NPs) and yttrium oxide (Y2O3NPs) nanoparticles are prevalent in many industries, including food and medicine, but their small size raises concerns about potential cellular damage and genotoxic effects. However, there are very limited studies available on their genotoxic effects. Hence, this was done to investigate the effects of multiple administration of Ca(OH)2NPs, CaTiO3NPs or/and Y2O3NPs on genomic DNA stability, mitochondrial membrane potential integrity and inflammation induction in mouse brain tissues. Mice were orally administered Ca(OH)2NPs, CaTiO3NPs or/and Y2O3NPs at a dose level of 50 mg/kg b.w three times a week for 2 weeks. Genomic DNA integrity was studied using Comet assay and the level of reactive oxygen species (ROS) within brain cells was analyzed using 2,7 dichlorofluorescein diacetate dye. The expression level of Presenilin-1, tumor necrosis factor-alpha (TNF-α) and Interleukin-6 (IL-6) genes and the integrity of the mitochondrial membrane potential were also detected. Oral administration of Ca(OH)2NPs caused the highest damage to genomic DNA and mitochondrial membrane potential, less genomic DNA and mitochondrial damage was induced by CaTiO3NPs administration while administration of Y2O3NPs did not cause any remarkable change in the integrity of genomic DNA and mitochondrial membrane potential. Highest ROS generation and upregulation of presenilin-1, TNF-α and IL-6 genes were also observed within the brain cells of mice administrated Ca(OH)2NPs but Y2O3NPs administration almost caused no changes in ROS generation and genes expression compared to the negative control. Administration of CaTiO3NPs alone slightly increased ROS generation and the expression level of TNF-α and IL-6 genes. Moreover, no remarkable changes in the integrity of genomic DNA and mitochondrial DNA potential, ROS level and the expression level of presenilin-1, TNF-α and IL-6 genes were noticed after simultaneous coadministration of Y2O3NPs with Ca(OH)2NPs and CaTiO3NPs. Coadministration of Y2O3NPs with Ca(OH)2NPs and CaTiO3NPs mitigated Ca(OH)2NPs and CaTiO3NPs induced ROS generation, genomic DNA damage and inflammation along with restoring the integrity of mitochondrial membrane potential through Y2O3NPs scavenging free radicals ability. Therefore, further studies are recommended to study the possibility of using Y2O3NPs to alleviate Ca(OH)2NPs and CaTiO3NPs induced genotoxic effects.
Funder
Faculty of Science, Cairo University
Cairo University
Publisher
Springer Science and Business Media LLC
Reference34 articles.
1. Kumar, M. S. Nano Science and Nanotechnology: Journey from Past to Present and Prospect in Veterinary Science and Medicine. Nanoscience 2(1), 79–83 (2014).
2. Abiodun-Solanke, I., Ajayi, D. & Arigbede, A. Nanotechnology and its application in dentistry. Ann. Med. Health Sci. Res. 3, S171–S177 (2014).
3. Jacobsen, N. R. et al. Genotoxicity, cytotoxicity, and reactive oxygen species induced by single-walled carbon nanotubes and C(60) fullerenes in the FE1-Mutatrade markMouse lung epithelial cells. Environ. Mol. Mutagen. 49(6), 476–478 (2008).
4. Ambrosi, M., Dei, L., Giorgi, R., Neto, C. & Baglioni, P. Colloidal particles of Ca(OH)2: Properties and applications to restoration of frescoes. Langmuir 17(14), 4251–4255 (2001).
5. Maroneze, M., Zepka, L., Vieira, J., Queiroz, M. & JacobLopes, E. A tecnologia de remoção de fósforo: Gerenciamento do elemento em resíduos industriais. Ambiente e Agua 9, 445–558 (2014).