Author:
Staats Kevin,Pilz Magdalena,Sun Jie,Boiadjieva-Scherzer Tzvetanka,Kronberger Hermann,Tobudic Selma,Windhager Reinhard,Holinka Johannes
Abstract
AbstractTitanium nanotube surfaces containing silver, zinc, and copper have shown antimicrobial effects without decreasing osteoblastic cell growth. In this in-vitro study we present first results on the biological evaluation of surface modifications by incorporating selenium and silver compounds into titanium-dioxide (TiO2) nanotubes by electrochemical deposition. TiO2-nanotubes (TNT) and Phosphate-doped TNT (pTNT) were grown on the surface of Ti6Al4V discs by anodization. Hydroxyapatite (HA), selenium (Se) and silver (Ag) compounds were incorporated by electrochemical deposition. Colony forming units of Staphylococcus epidermidis (DSM 3269) were significantly decreased in SepTNT (0.97 ± 0.18 × 106 CFU/mL), SepTNT-HA (1.2 ± 0.39 × 106 CFU/mL), AgpTNT (1.36 ± 0.42 × 106 CFU/mL) and Ag2SepTNT (0.999 ± 0.12 × 106 CFU/mL) compared to the non-modified control (2.2 ± 0.21 × 106 CFU/mL). Bacterial adhesion was calculated by measuring the covered area after fluorescence staining. Adhesion was lower in SepTNT (37.93 ± 12%; P = 0.004), pTNT (47.3 ± 6.3%, P = 0.04), AgpTNT (24.9 ± 1.8%; P < 0.001) and Ag2SepTNT (14.9 ± 4.9%; P < 0.001) compared to the non-modified control (73.7 ± 11%). Biofilm formation and the growth of osteoblastic cells (MG-63) was observed by using Crystal Violet staining. Biofilm formation was reduced in SepTNT (22 ± 3%, P = 0.02) and Ag2SepTNT discs (23 ± 11%, P = 0.02) compared to the non-modified control (54 ± 8%). In comparison with the non-modified control the modified SepTNT-HA and pTNT surfaces showed a significant higher covered area with osteoblastic MG-63-cells. Scanning electron microscope (SEM) images confirmed findings regarding bacterial and osteoblastic cell growth. These findings show a potential synergistic effect by combining selenium and silver with titanium nanotubes.
Publisher
Springer Science and Business Media LLC
Reference41 articles.
1. Kurtz, S., Ong, K., Lau, E., Mowat, F. & Halpern, M. Projections of primary and revision hip and knee arthroplasty in the United States from 2005 to 2030. J. Bone Joint Surg. Am. 89, 780–785 (2007).
2. Kurtz, S. M. et al. Are we winning or losing the battle with periprosthetic joint infection: Trends in periprosthetic joint infection and mortality risk for the medicare population. J. Arthroplasty 33, 3238–3245 (2018).
3. Kurtz, S., Lau, E., Watson, H., Schmier, J. & Parvizi, J. Economic burden of periprosthetic joint infection in the United States. J. Arthroplasty 27, 61–5.e1 (2012).
4. Karczewski, D. et al. A standardized interdisciplinary algorithm for the treatment of prosthetic joint infections. Bone Joint J. 101-B, 132–139 (2019).
5. Middleton, R., Khan, T. & Alvand, A. Update on the diagnosis and management of prosthetic joint infection in hip and knee arthroplasty. Bone Jt. 360(8), 5–13 (2019).
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献