Abstract
AbstractIn the neural progenitors of the developing central nervous system
(CNS), cell proliferation is tightly controlled and coordinated with cell fate
decisions. Progenitors divide rapidly during early development and their cell cycle
lengthens progressively as development advances to eventually give rise to a tissue
of the correct size and cellular composition. However, our understanding of the
molecules linking cell cycle progression to developmental time is incomplete. Here,
we show that the microRNA (miRNA) let-7 accumulates in neural progenitors over time
throughout the developing CNS. Intriguingly, we find that the level and activity of
let-7 oscillate as neural progenitors progress through the cell cycle by in situ hybridization and fluorescent miRNA sensor
analyses. We also show that let-7 mediates cell cycle dynamics: increasing the level
of let-7 promotes cell cycle exit and lengthens the S/G2 phase of the cell cycle,
while let-7 knock down shortens the cell cycle in neural progenitors. Together, our
findings suggest that let-7 may link cell proliferation to developmental time and
regulate the progressive cell cycle lengthening that occurs during
development.
Funder
U.S. Department of Health
& Human Services | NIH | National Eye Institute
U.S. Department of Health
& Human Services | NIH | National Institute of Neurological Disorders and
Stroke
U.S. Department of Health & Human Services | NIH | National Eye
Institute
Publisher
Springer Science and Business Media LLC
Cited by
28 articles.
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