Abstract
AbstractBud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, which seems to be involved in the development of BN.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Ministry of Science, Technology and Innovation | Conselho Nacional de Desenvolvimento Científico e Tecnológico
Publisher
Springer Science and Business Media LLC
Reference53 articles.
1. Cox, C. M., Favero, A. C., Dry, P. R., McCarthy, M. G. & Collins, C. Rootstock Effects on Primary Bud Necrosis, Bud Fertility, and Carbohydrate Storage in Shiraz. Am. J. Enol. Vitic. 63, 277 LP–283 (2012).
2. Collins, C. & Rawnsley, B. Factors influencing primary bud necrosis (PBN) in Australian vineyards. Acta Hortic. 689, 81–86 (2005).
3. Wolf, T. K. & Warren, M. K. Shoot Growth Rate and Density Affect Bud Necrosis of ‘Riesling’ Grapevines. J. Am. Soc. Hortic. Sci. 120, 989–996 (1995).
4. Naito, R., Yamamura, H. & Yoshino, K. Effects of shoot vigor and foliar application of GA and SADH on the occurrence of bud necrosis in ‘Kyoho’grape. J. Japanese Soc. Hortic. Sci. 55, 130–137 (1986).
5. Ahmadi, J., Abedy, B., Shoor, M., Eshghi, S. & Fahliani, R. A. Effect of salicylic acid and potassium sulfate on the primary bud necrosis and fruit set of the following year of Askari grapevine. EurAsian J. Biosci. 12, 39–47 (2018).
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献