Abstract
AbstractPeriodontitis is a prevalent chronic inflammatory disease due to the host response (IL-1β, IL-6, TNF-α and IL-17A) to oral bacteria such as Porphyromonas gingivalis. The newer members of the IL-1 family, IL-36s (IL-36α/IL-36β/IL-36γ/IL-36Ra/IL-38) are known to be involved in host defense against P. gingivalis in oral epithelial cells (OECs) and are considered as key inflammatory mediators in chronic diseases. The aim of this study was to investigate the potential role of IL-36s in periodontitis. We showed here that IL-36γ mRNA gingival expression is higher in periodontitis patients, whereas IL-36β and IL-36Ra mRNA expression are lower compared to healthy controls. Interestingly, the elevated IL-36γ expression in patients is positively correlated with the RANKL/OPG ratio, an index of bone resorption. In vitro, IL-36γ expression was induced through TLR2 activation in primary OECs infected with P. gingivalis but not in gingival fibroblasts, the most widespread cell type in gingival connective tissue. In OECs, recombinant IL-36γ enhanced the expression of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-36γ), of TLR2 and importantly, the RANKL/OPG ratio. These findings suggest that IL-36γ could be a pivotal inflammatory player in periodontitis by perpetuating gingival inflammation and its associated alveolar bone resorption and could be a relevant therapeutic target.
Funder
Institut Français pour la Recherche Odontologique (IFRO), RFI Bioregate (Région Pays de la Loire) (Inflamos 38 Project) and the School of Dentistry of Nantes.
Publisher
Springer Science and Business Media LLC
Reference43 articles.
1. Papapanou, P. N. et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri‐ Implant Diseases and Conditions. J. Periodontol. 45, 286–291 (2018).
2. Löe, H. The sixth complication of diabetes mellitus. Diabetes Care 16, 329–334 (1993).
3. Tang, Q. et al. A Possible Link Between Rheumatoid Arthritis and Periodontitis: A Systematic Review and Meta-analysis. Int. J. Periodontics Restorative Dent. 37, 79–86 (2017).
4. Almeida, A. P. C. P. S. C., Fagundes, N. C. F., Maia, L. C. & Lima, R. R. Is There An Association Between Periodontitis And Atherosclerosis In Adults? A Systematic Review. Curr. Vasc. Pharmacol. 1–2 (2017).
5. Moraschini, V., de Albuquerque Calasans-Maia, J. & Diuana Calasans-Maia, M. Association Between Asthma and Periodontal Disease: A Systematic Review and Meta-Analysis. J. Periodontol. 1–20 (2017).
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