Author:
Sina Rahme Bechara,Lestradet Matthieu,Di Venanzio Gisela,Ayyaz Arshad,Yamba Miriam Wennida,Lazzaro Martina,Liégeois Samuel,Garcia Véscovi Eleonora,Ferrandon Dominique
Abstract
AbstractSerratia marcescens is an opportunistic bacterium that infects a wide range of hosts including humans. It is a potent pathogen in a septic injury model of Drosophila melanogaster since a few bacteria directly injected in the body cavity kill the insect within a day. In contrast, flies do not succumb to ingested bacteria for days even though some bacteria cross the intestinal barrier into the hemolymph within hours. The mechanisms by which S. marcescens attacks enterocytes and damages the intestinal epithelium remain uncharacterized. To better understand intestinal infections, we performed a genetic screen for loss of virulence of ingested S. marcescens and identified FliR, a structural component of the flagellum, as a virulence factor. Next, we compared the virulence of two flagellum mutants fliR and flhD in two distinct S. marcescens strains. Both genes are required for S. marcescens to escape the gut lumen into the hemocoel, indicating that the flagellum plays an important role for the passage of bacteria through the intestinal barrier. Unexpectedly, fliR but not flhD is involved in S. marcescens-mediated damages of the intestinal epithelium that ultimately contribute to the demise of the host. Our results therefore suggest a flagellum-independent role for fliR in bacterial virulence.
Funder
Agence Nationale de la Recherche
Université de Strasbourg
ECOS-Sud (Ministère des Affaires Etrangères de France)-MINCT (Ministerio de Cienca y Technologia e Innovacion, Argentina) exchange program #A12B04
Agencia Nacional de Promoción Científica y Tecnológica
Consejo Nacional de Investigaciones Científicas y Técnicas
Centre National de la Recherche Scientifique
Higher Education Commision, Pakistan
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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