Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design
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Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-019-47376-1.pdf
Reference39 articles.
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2. Reetz, M. T. et al. Expanding the substrate scope of enzymes: combining mutations obtained by CASTing. Chemistry 12, 6031–6038, https://doi.org/10.1002/chem.200600459 (2006).
3. Reetz, M. T. & Carballeira, J. D. Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Nat Protoc 2, 891–903, https://doi.org/10.1038/nprot.2007.72 (2007).
4. Acevedo-Rocha, C. G., Hoebenreich, S. & Reetz, M. T. Iterative saturation mutagenesis: a powerful approach to engineer proteins by systematically simulating Darwinian evolution. Methods Mol Biol 1179, 103–128, https://doi.org/10.1007/978-1-4939-1053-3_7 (2014).
5. Chiang, L. W., Kovari, I. & Howe, M. M. Mutagenic oligonucleotide-directed PCR amplification (Mod-PCR): an efficient method for generating random base substitution mutations in a DNA sequence element. PCR Methods Appl 2, 210–217 (1993).
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