Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture
Author:
Funder
Funding by the internal program (HiLF) of the Hannover Medical School.
Deutsche Forschungsgemeinschaft
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-018-37650-z.pdf
Reference52 articles.
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3. Naujok, O., Burns, C., Jones, P. M. & Lenzen, S. Insulin-producing surrogate beta-cells from embryonic stem cells: are we there yet? Mol Ther 19, 1759–1768 (2011).
4. Diekmann, U., Lenzen, S. & Naujok, O. A reliable and efficient protocol for human pluripotent stem cell differentiation into the definitive endoderm based on dispersed single cells. Stem Cells Dev 24, 190–204 (2015).
5. Naujok, O., Diekmann, U. & Lenzen, S. The generation of definitive endoderm from human embryonic stem cells is initially independent from activin A but requires canonical Wnt-signaling. Stem Cell Rev 10, 480–493 (2014).
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