Author:
Zhang Shikai,Zhan Wang,Sun Anran,Xie Ying,Han Zhiming,Qu Xibin,Wang Jiayi,Zhang Laifu,Tian Mingshun,Pang Xuhong,Zhang Jinbao,Zhao Xiyang
Abstract
AbstractThe red color formation of Acer mandshuricum leaves is caused by the accumulation of anthocyanins primarily, but the molecular mechanism researches which underlie anthocyanin biosynthesis in A. mandshuricum were still lacking. Therefore, we combined the transcriptome and metabolome and analyzed the regulatory mechanism and accumulation pattern of anthocyanins in three different leaf color states. In our results, 26 anthocyanins were identified. Notably, the metabolite cyanidin 3-O-glucoside was found that significantly correlated with the color formation, was the predominant metabolite in anthocyanin biosynthesis of A. mandshuricum. By the way, two key structural genes ANS (Cluster-20561.86285) and BZ1 (Cluster-20561.99238) in anthocyanidin biosynthesis pathway were significantly up-regulated in RL, suggesting that they might enhance accumulation of cyanidin 3-O-glucoside which is their downstream metabolite, and contributed the red formation of A. mandshuricum leaves. Additionally, most TFs (e.g., MYBs, bZIPs and bHLHs) were detected differentially expressed in three leaf color stages that could participate in anthocyanin accumulation. This study sheds light on the anthocyanin molecular regulation of anthocyanidin biosynthesis and accumulation underlying the different leaf color change periods in A. mandshuricum, and it could provide basic theory and new insight for the leaf color related genetic improvement of A. mandshuricum.
Funder
Heilongjiang Province Applied Technology Research and Development Plan Project
Fundamental Research Funds for the Central Universities
Heilongjiang Touyan Innovation Team Program
Publisher
Springer Science and Business Media LLC
Reference69 articles.
1. Zhu, Y. Y. Research on relationships between functional traits and compensation mechanisms of androdioecious Acer mandshuricum, master thesis, Beijing Forestry University (2016).
2. Liu, X. L. Research on red pigment in leaves and leaves color retention of Acer mandshuricum, master thesis, Northeast Forestry University (2014).
3. Xie, Z., Yi, X. Y. & Xia, F. C. The resource situation and development utilization of Acer plants in northeast area. J. Jilin For. Sci. Technol. 46, 26-31+47. https://doi.org/10.16115/j.cnki.issn.1005-7129.2017.01.007 (2017).
4. Reay, P. F., Fletcher, R. H. & Thomas, V. J. Chlorophylls, carotenoids and anthocyanin concentrations in the skin of “Gala” apples during maturation and the influence of foliar applications of nitrogen and magnesium. J. Sci. Food Agric. 76, 63–71. https://doi.org/10.1002/(SICI)1097-0010(199801)76:1 (2015).
5. Englert, M., Hammann, S. & Vetter, W. Isolation of β-carotene, α-carotene and lutein from carrots by countercurrent chromatography with the solvent system modifier benzotrifluoride. J. Chromatogr. A 1388, 119–125. https://doi.org/10.1016/j.chroma.2015.02.020 (2015).