Author:
Okumura Takamasa,Attri Pankaj,Kamataki Kunihiro,Yamashita Naoto,Tsukada Yuichi,Itagaki Naho,Shiratani Masaharu,Ishibashi Yushi,Kuchitsu Kazuyuki,Koga Kazunori
Abstract
AbstractDischarge plasma irradiates seeds with reactive oxygen and nitrogen species (RONS). However, RONS introduced in seeds by plasma irradiation have not been successfully detected thus far. This study provides experimental evidence that nitrate ion NO3− is introduced in lettuce seeds as RONS upon irradiation with atmospheric-pressure air dielectric barrier discharge plasma. Plasma irradiation for 5 min promotes seed germination. The components of the plasma-irradiated seeds were examined using electrospray ionization quantum mass spectrometry (ESI QMS), which revealed that the plasma irradiation introduced an ion with a mass of 62 m/z in detectable amounts. This ion was identified as NO3− by liquid chromatography (LC), multiple wavelength detector (MWD), and LC-ESI QMS. A one-dimensional simulation at electron temperature Te = 1 eV, electron density Ne = 1013/m3, and gas temperature Tg = 300 K indicated the introduction of NO3−, involving nitric oxide NO. NO3− is one of the most important ions that trigger signal transduction for germination when introduced in seeds. The scanning electron microscopy (SEM) images revealed that there was no change on the surface of the seeds after plasma irradiation. Plasma irradiation is an effective method of introducing NO3− in seeds in a dry process without causing damage.
Funder
QR Program
Japan Society for the Promotion of Science
Publisher
Springer Science and Business Media LLC
Cited by
5 articles.
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