Author:
Fariha Ramisa,Deshpande Prutha S.,Rothkopf Emma,Jabrah Mohannad,Spooner Adam,Okoh Oluwanifemi David,Tripathi Anubhav
Abstract
AbstractDepression is a growing global crisis, with females at a higher rate of diagnosis than males. While the percentage of patients on prescribed antidepressants have tripled over the last two decades, we are still at a crossroad where the discrepancy lies between finding a drug to suit a patient and monitoring the abundance of it in the body to prevent unwanted side effects. Liquid Chromatography tandem mass spectrometry (LC–MS/MS) has garnered the attention of clinicians as a technique to accurately monitor therapeutic drugs in human serum with high specificity and accuracy. This may be a potential solution, but the challenge persists in the realm of sample preparation, where a method is automatable. We have developed and validated an LC–MS/MS-based assay for simultaneous quantification of 4 different classes of commonly prescribed antidepressants in women that is automated using a JANUS G3 Robotic Liquid Handler. Our method utilizes a simple sample preparation technique, utilizing only 20 μL of a serum sample, to accurately measure Bupropion, Citalopram, Desipramine, Imipramine, Olanzapine, Sertraline and Vilazodone across a range of 1.0 to 230 ng/mL. Our method exhibits a linearity of R2 ≥ 0.99 when detected in MRM mode and % CV of ≤ 20% for all analytes across the board. In addition, we have designed a prototype that can be utilized at a clinical mass spectrometry lab and assessed the long-term use of this prototype using an accelerated stability study. Overall, our developed method has the potential to be translated to clinical settings to monitor postpartum depression for a large number of patient samples using automation.
Publisher
Springer Science and Business Media LLC
Reference34 articles.
1. Depression [Internet]. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/depression (Accessed 12 October 2022).
2. SAMHSA - Substance abuse and mental health services administration. https://www.samhsa.gov/ (Accessed 12 October 2022).
3. Treatment [Internet]. Depression and bipolar support alliance. https://www.dbsalliance.org/wellness/treatment-options/therapy/ (Accessed 12 October 2022).
4. Winerman L. By the numbers: Antidepressant use on the rise [Internet]. American Psychological Association. https://www.apa.org/monitor/2017/11/numbers (Accessed 12 October 2022).
5. Bogowicz, P. et al. Trends and variation in antidepressant prescribing in English primary care: A retrospective longitudinal study. BJGP Open 5(4), BJGPO.2021.0020 (2021).
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