Abstract
AbstractNovel H1N2 influenza A viruses (IAVs) in swine have been identified in Chile co-circulating with pandemic H1N1 2009-like (A(H1N1)pdm09-like) viruses. The objective of this study was to characterize antigenically the swine H1 IAVs circulating in Chile. Genetic analysis based on the HA1 domain and antigenic analysis by hemagglutination inhibition assay were carried out. Three antigenic clusters were identified, named Chilean H1 A (ChH1A), Chilean H1 B (ChH1B), and A(H1N1)pdm09-like. The antigenic sites of ChH1A and ChH1B strains were 10–60% distant from those of commercial vaccine strains at the amino acid sequence level. Antigenic variants were identified within the clusters ChH1A and A(H1N1)pdm09-like. Substitutions in the main antigenic sites (E153G in Sa, Q193H in Sb, D168N in Ca1, P137S in Ca2, and F71L in Cb) were detected in variants from the ChH1A cluster, whereas only a single substitution in antigenic site Sa (G155E) was detected in variants from A(H1N1)pdm09-like cluster, which confirms the importance to carrying out antigenic analyses in addition to genetic analyses to evaluate control measures such as vaccination. These results highlight the need to update vaccines for swine in Chile and the importance of continued surveillance to determine the onward transmission of antigenic variants in Chilean pig populations.
Funder
Consejo Nacional de Innovación, Ciencia y Tecnología
U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases
Publisher
Springer Science and Business Media LLC
Reference56 articles.
1. International Committee on Taxonomy of Viruses (ICTV). Virus Taxonomy: 2018b Release. Preprint at, https://talk.ictvonline.org/taxonomy/ (2019).
2. Tong, S. et al. New world bats harbor diverse influenza A viruses. Plos Pathog. 9, e1003657, https://doi.org/10.1371/journal.ppat.1003657 (2013).
3. Scholtissek, C., Bürger, H., Kistner, O. & Shortridge, K. F. The nucleoprotein as a possible major factor in determining host specificity of influenza H3N2 viruses. Virology 147, 287–294 (1985).
4. Joseph, U., Su, Y. C., Vijaykrishna, D. & Smith, G. J. The ecology and adaptive evolution of influenza A interspecies transmission. Influenza Other Respir. Viruses 11, 74–84 (2017).
5. Smith, G. J. et al. Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature 459, 1122–1125 (2009).
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