Author:
Li Nanfen,Yu Jun,Yang Jinpeng,Wang Sheliang,Yu Lianying,Xu Fangsen,Yang Chunlei
Abstract
AbstractCigar variety CX-010 tobacco leaves produce localized green spots during the air-curing period, and spraying exogenous sucrose effectively alleviates the occurrence of the green spots. To investigate the alleviation effect of exogenous sucrose spraying, the total water content and the number and size of green spots on tobacco leaves were investigated during the air-curing period under four treatments; CK (pure water), T1 (0.1 M sucrose), T2 (0.2 M sucrose) and T3 (0.4 M sucrose). The results showed that the total water content of tobacco leaves showed a trend of T3 < CK < T2 < T1 in the early air-curing stage, and the number and size of green spots showed a trend of T3 < T2 < T1 < CK. All sucrose treatments alleviated the green spot phenomenon, and T3 had the fewest green spots. Thus, the tobacco leaves of the T3 and CK treatments at two air-curing stages were used to perform metabolomics analysis with nontargeted liquid chromatography‒mass spectrometry to determine the physiological mechanism. A total of 259 and 178 differentially abundant metabolites (DAMs) between T3- and CK-treated tobacco leaves were identified in the early air-curing and the end of air-curing stages, respectively. These DAMs mainly included lipid and lipid-like molecules, carbohydrates, and organic acids and their derivatives. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the T3 treatment significantly altered carbohydrate metabolism (pentose phosphate pathway, sucrose and starch metabolism and galactose metabolism) and amino acid metabolism (tyrosine metabolism and tryptophan metabolism) in air-curing tobacco leaves. Sucrose treatment alleviated green spots by altering DAMs that affected chlorophyll degradation, such as tyrosine and citric acid, to promote the normal degradation of chlorophyll.
Funder
the Key Science and Technology Project of China National Tobacco Corporation
the Key Science and Technology Project of Hubei Branch of China National Tobacco Corporation
Publisher
Springer Science and Business Media LLC
Reference38 articles.
1. Cai, B. et al. Research progress of production technologies of cigar tobaccos in China. Acta Tab. Sin. 25, 110–119 (2019).
2. Gong, C. R. Tobacco Curing Science (ed. Gong, C. R.) 280–284 (China Agriculture Press, 2011).
3. Andersen, R. A., Kasperbauer, M. J. & Burton, H. R. Shade during growth—effects on chemical composition and leaf color of air-cured burley tobacco1. Agron J. 77, 543–546 (1985).
4. Yu, et al. Metabolomic analysis of metabolic differences and causes of green spot tissue of cigar tobacco during air-curing. J. Huazhong Agric. Univ. 41, 152–159 (2022).
5. Jia, H. F. et al. Sucrose functions as a signal involved in the regulation of strawberry fruit development and ripening. New Phytol. 198, 453–465 (2013).
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献