Reproduction of bacterial chemotaxis by a non-living self-propelled object

Abstract

AbstractTaxic behavior as a response to an external stimulus is a fundamental function of living organisms. Some bacteria successfully implement chemotaxis without directly controlling the direction of movement. They periodically alternate between run and tumble, i.e., straight movement and change in direction, respectively. They tune their running period depending on the concentration gradient of attractants around them. Consequently, they respond to a gentle concentration gradient stochastically, which is called “bacterial chemotaxis.” In this study, such a stochastic response was reproduced by a non-living self-propelled object. We used a phenanthroline disk floating on an aqueous solution of Fe$$^{2+}$$ 2 + . The disk spontaneously alternated between rapid motion and rest, similar to the run-and-tumble motion of bacteria. The movement direction of the disk was isotropic independent of the concentration gradient. However, the existing probability of the self-propelled object was higher at the low-concentration region, where the run length was longer. To explain the mechanism underlying this phenomenon, we proposed a simple mathematical model that considers random walkers whose run length depends on the local concentration and direction of movement against the gradient. Our model adopts deterministic functions to reproduce the both effects, which is instead of stochastic tuning the period of operation used in the previous reports. This allows us to analyze the proposed model mathematically, which indicated that our model reproduces both positive and negative chemotaxis depending on the competition between the local concentration effect and it’s gradient effect. Owing to the newly introduced directional bias, the experimental observations were reproduced numerically and analytically. The results indicate that the directional bias response to the concentration gradient is an essential parameter for determining bacterial chemotaxis. This rule might be universal for the stochastic response of self-propelled particles in living and non-living systems.