Author:
Copilaş-Ciocianu Denis,Rewicz Tomasz,Sands Arthur F.,Palatov Dmitry,Marin Ivan,Arbačiauskas Kęstutis,Hebert Paul D. N.,Grabowski Michal,Audzijonyte Asta
Abstract
AbstractThe Ponto-Caspian region is an endemicity hotspot that harbours several crustacean radiations, among which amphipods are the most diverse. These poorly known species are severely threatened in their native range, while at the same time they are invading European inland waters with significant ecological consequences. A proper taxonomic knowledge of this fauna is paramount for its conservation within the native region and monitoring outside of it. Here, we assemble a DNA barcode reference library for nearly 60% of all known Ponto-Caspian amphipod species. We use several methods to define molecular operational taxonomic units (MOTUs), based on two mitochondrial markers (COI and 16S), and assess their congruence with current species-level taxonomy based on morphology. Depending on the method, we find that 54–69% of species had congruent morpho-molecular boundaries. The cases of incongruence resulted from lumping distinct morphospecies into a single MOTU (7–27%), splitting a morphospecies into several MOTUs (4–28%), or both (4–11%). MOTUs defined by distance-based methods without a priori divergence thresholds showed the highest congruence with morphological taxonomy. These results indicate that DNA barcoding is valuable for clarifying the diversity of Ponto-Caspian amphipods, but reveals that extensive work is needed to resolve taxonomic uncertainties. Our study advances the DNA barcode reference library for the European aquatic biota, paving the way towards improved taxonomic knowledge needed to enhance monitoring and conservation efforts.
Funder
Lietuvos Mokslo Taryba
Narodowym Centrum Nauki
Narodowa Agencja Wymiany Akademickiej
European Commission
New Frontiers in Research Fund
Publisher
Springer Science and Business Media LLC
Cited by
16 articles.
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