Author:
Jiao Lihe,Han Chuang,Zhu Jianan,Zhang Piqi,Ma Yinpeng,Dai Xiaodong,Zhang Yunzhi
Abstract
AbstractAuricularia heimuer, the third most frequently cultivated edible mushroom species worldwide, has high medicinal value. However, a shortage of molecular marker hinders the efficiency and accuracy of genetic breeding efforts for A. heimuer. High-throughput transcriptome sequencing data are essential for gene discovery and molecular markers development. This study aimed to clarify the distribution of SSR loci across the A. heimuer transcriptome and to develop highly informative EST-SSR markers. These tools can be used for phylogenetic analysis, functional gene mining, and molecular marker-assisted breeding of A. heimuer. This study used Illumina high-throughput sequencing technology to obtain A. heimuer transcriptome data. The results revealed 37,538 unigenes in the A. heimuer transcriptome. Of these unigenes, 24,777 (66.01%) were annotated via comparison with the COG, Pfam, and NR databases. Overall, 2510 SSRs were identified from the unigenes, including 6 types of SSRs. The most abundant type of repeats were trinucleotides (1425, 56.77%), followed by mononucleotides (391, 15.58%) and dinucleotides (456, 18.17%). Primer pairs for 102 SSR loci were randomly designed for validity confirmation and polymorphism identification; this process yielded 53 polymorphic EST-SSR markers. Finally, 13 pairs of highly polymorphic EST-SSR primers were used to analyze the genetic diversity and population structure of 52 wild A. heimuer germplasms, revealing that the 52 germplasms could be divided into three categories. These results indicated that SSR loci were abundant in types, numbers, and frequencies, providing a potential basis for germplasm resource identification, genetic diversity analysis, and molecular marker-assisted breeding of A. heimuer.
Funder
special funds of scientific research business fee project of Heilongjiang scientific research insititution
Publisher
Springer Science and Business Media LLC
Reference54 articles.
1. Wu, F., Yuan, Y., Malysheva, V., Du, P. & Dai, Y.-C. Species clarification of the most important and cultivated Auricularia mushroom “Heimuer”: Evidence from morphological and molecular data. Phytotaxa 186, 241–253 (2014).
2. Wu, Z. C. Preliminary Study on the Biosynthesis of Main Active Ingredients of Auricuralia auricular. Wenzhou University (2018).
3. Sun, X. D. et al. Analysis of selemium content in common and selenium enriched Auricularia auricular and Lentinus edodes in China. Qual. Saf. Agroprod 01, 38–41 (2021).
4. Ito, V. C. & Lacerda, L. G. Black rice (Oryza sativa L.): A review of its historical aspects, chemical composition, nutritional and functional properties, and applications and processing technologies. Food Chem. 301, 125304. https://doi.org/10.1016/j.foodchem.2019.125304 (2019).
5. Reza, M. A., Jo, W. S. & Park, S. C. Comparative antitumor activity of jelly ear culinary-medicinal mushroom, Auricularia auricula-judae (Bull.) J. Schrot. (higher basidiomycetes) extracts against tumor cells in vitro. Int. J. Med. Mushrooms 14, 403–409. https://doi.org/10.1615/intjmedmushr.v14.i4.80 (2012).