Author:
Zainol Abidin Intan Zarina,Fazry Shazrul,Jamar Nur Hidayah,Ediwar Dyari Herryawan Ryadi,Zainal Ariffin Zaidah,Johari Anis Nabilah,Ashaari Nur Suhanawati,Johari Nor Azfa,Megat Abdul Wahab Rohaya,Zainal Ariffin Shahrul Hisham
Abstract
AbstractIn Malaysia, Piper sarmentosum or ‘kaduk’ is commonly used in traditional medicines. However, its biological effects including in vivo embryonic toxicity and tissue regenerative properties are relatively unknown. The purpose of this study was to determine zebrafish (Danio rerio) embryo toxicities and caudal fin tissue regeneration in the presence of P. sarmentosum aqueous extracts. The phytochemical components and antioxidant activity of the extract were studied using GC–MS analysis and DPPH assay, respectively. Embryo toxicity tests involving survival, heartbeat, and morphological analyses were conducted to determine P. sarmentosum extract toxicity (0–60 µg/mL); concentrations of 0–400 µg/mL of the extract were used to study tissue regeneration in the zebrafish caudal fin. The extract contained several phytochemicals with antioxidant activity and exhibited DPPH scavenging activity (IC50 = 50.56 mg/mL). Embryo toxicity assays showed that a concentration of 60 μg/mL showed the highest rates of lethality regardless of exposure time. Slower embryogenesis was observed at 40 µg/mL, with non-viable embryos first detected at 50 µg/mL. Extracts showed significant differences (p < 0.01) for tissue regeneration at all concentrations when compared to non-treated samples. In conclusion, Piper sarmentosum extracts accelerated tissue regeneration, and extract concentrations at 60 µg/mL showed the highest toxicity levels for embryo viability.
Publisher
Springer Science and Business Media LLC
Reference54 articles.
1. Horcajada, M. N. et al. Hesperidin inhibits ovariectomized-induced osteopenia and shows differential effects on bone mass and strength in young and adult intact rats. J. Appl. Physiol. 104, 648–654 (2008).
2. Shahrul Hisham, Z. A. et al. Intrinsic anticarcinogenic effects of Piper sarmentosum ethanolic extract on a human hepatoma cell line. Cancer Cell Int. 9, 6 (2009).
3. Wan Haifa Haryani, W. O. et al. Anticancer Screening of Ethanol Extract from Selected Piperaceae Family and its Determination via Trypan Blue Staining. SAINS MALAYSIANA. 39, 941–949 (2010).
4. Masters, J. R. et al. Short tandem repeat profiling provides an international reference standard for human cell lines. Proc. Natl. Acad. Sci. 98, 8012–8017 (2001).
5. Rahman, N. et al. Piper sarmentosum influences the oxidative stress involved in experimental diabetic rats. Internet J. Herb. Plant Med. 1, 1 (2011).
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献