Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

Author:

Lloyd Charmaine,Tan Kai Heng,Lim Kar Leong,Valu Vimala Gana,Fun Sarah Mei Ying,Chye Teng Rong,Mak Hui Min,Sim Wei Xiong,Musa Sarah  Liyana,Ng Joscelyn Jun Quan,Bte Nordin Nazurah Syazana,Bte Md Aidzil Nurhazlyn,Eng Zephyr Yu Wen,Manickavasagam Punithavathy,New Jen Yan

Abstract

AbstractThis study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, EH = 0.6), of whichScenedesmusspp. andChlorellaspp. constituted 27.5% and 21.3% of isolates respectively, followed byMicractiniumspp. (18.8%) andChlamydomonasspp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.

Funder

Tote board - Singapore

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Reference50 articles.

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