Author:
Jalal Khurshid,Abu-Izneid Tareq,Khan Kanwal,Abbas Muhammad,Hayat Ajmal,Bawazeer Sami,Uddin Reaz
Abstract
AbstractShigellosis is characterized as diarrheal disease that causes a high mortality rate especially in children, elderly and immunocompromised patients. More recently, the World Health Organization advised safe vaccine designing against shigellosis due to the emergence of Shigella dysenteriae resistant strains. Therefore, the aim of this study is to identify novel drug targets as well as the design of the potential vaccine candidates and chimeric vaccine models against Shigella dysenteriae. A computational based Reverse Vaccinology along with subtractive genomics analysis is one of the robust approaches used for the prioritization of drug targets and vaccine candidates through direct screening of genome sequence assemblies. Herein, a successfully designed peptide-based novel highly antigenic chimeric vaccine candidate against Shigella dysenteriae sd197 strain is proposed. The study resulted in six epitopes from outer membrane WP_000188255.1 (Fe (3+) dicitrate transport protein FecA) that ultimately leads to the construction of twelve vaccine models. Moreover, V9 construct was found to be highly immunogenic, non-toxic, non-allergenic, highly antigenic, and most stable in terms of molecular docking and simulation studies against six HLAs and TLRS/MD complex. So far, this protein and multiepitope have never been characterized as vaccine targets against Shigella dysenteriae. The current study proposed that V9 could be a significant vaccine candidate against shigellosis and to ascertain that further experiments may be applied by the scientific community focused on shigellosis.
Funder
Higher Education Commision, Pakistan
International Foundation for Science
Publisher
Springer Science and Business Media LLC
Reference66 articles.
1. Killackey, S. A., Sorbara, M. T. & Girardin, S. E. Cellular aspects of Shigella pathogenesis: Focus on the manipulation of host cell processes. Front. Cell. Infect. Microbiol. 6, 38 (2016).
2. Kotloff, K. L. et al. Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): A prospective, case-control study. The Lancet 382, 209–222 (2013).
3. Halimeh, F. B. et al. Historical, current, and emerging tools for identification and serotyping of Shigella. Brazil. J. Microbiol. 1, 1–13 (2021).
4. Kotloff, K. L. et al. Global burden of Shigella infections: Implications for vaccine development and implementation of control strategies. Bull. World Health Organ. 77, 651 (1999).
5. Mani, S., Wierzba, T. & Walker, R. I. Status of vaccine research and development for Shigella. Vaccine 34, 2887–2894 (2016).
Cited by
18 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献