Author:
Bhatti Gaurav,Romero Roberto,Gomez-Lopez Nardhy,Pique-Regi Roger,Pacora Percy,Jung Eunjung,Yeo Lami,Hsu Chaur-Dong,Kavdia Mahendra,Tarca Adi L.
Abstract
AbstractThe amniotic fluid (AF) cell-free RNA was shown to reflect physiological and pathological processes in pregnancy, but its value in the prediction of spontaneous preterm delivery is unknown. Herein we profiled cell-free RNA in AF samples collected from women who underwent transabdominal amniocentesis after an episode of spontaneous preterm labor and subsequently delivered within 24 h (n = 10) or later (n = 28) in gestation. Expression of known placental single-cell RNA-Seq signatures was quantified in AF cell-free RNA and compared between the groups. Random forest models were applied to predict time-to-delivery after amniocentesis. There were 2385 genes differentially expressed in AF samples of women who delivered within 24 h of amniocentesis compared to gestational age-matched samples from women who delivered after 24 h of amniocentesis. Genes with cell-free RNA changes were associated with immune and inflammatory processes related to the onset of labor, and the expression of placental single-cell RNA-Seq signatures of immune cells was increased with imminent delivery. AF transcriptomic prediction models captured these effects and predicted delivery within 24 h of amniocentesis (AUROC = 0.81). These results may inform the development of biomarkers for spontaneous preterm birth.
Publisher
Springer Science and Business Media LLC
Reference124 articles.
1. WHO. ICD-11 for Mortality and Morbidity Statistics. https://icd.who.int/browse11/l-m/en#/http://id.who.int/icd/entity/1726201225 (2018).
2. Blencowe, H. et al. National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries: A systematic analysis and implications. Lancet 379, 2162–2172. https://doi.org/10.1016/S0140-6736(12)60820-4 (2012).
3. Liu, L. et al. Global, regional, and national causes of under-5 mortality in 2000–15: An updated systematic analysis with implications for the sustainable development goals. Lancet 388, 3027–3035. https://doi.org/10.1016/s0140-6736(16)31593-8 (2016).
4. Martin, J. A., Hamilton, B. E., Osterman, M. J. K. & Driscoll, A. K. Births: Final Data for 2018 (National Center for Health Statistics, 2019).
5. Brown, H. K., Speechley, K. N., Macnab, J., Natale, R. & Campbell, M. K. Neonatal morbidity associated with late preterm and early term birth: The roles of gestational age and biological determinants of preterm birth. Int. J. Epidemiol. 43, 802–814. https://doi.org/10.1093/ije/dyt251 (2014).
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献