Author:
Long Rory K. M.,Moriarty Kathleen P.,Cardoen Ben,Gao Guang,Vogl A. Wayne,Jean François,Hamarneh Ghassan,Nabi Ivan R.
Abstract
AbstractThe endoplasmic reticulum (ER) is a complex subcellular organelle composed of diverse structures such as tubules, sheets and tubular matrices. Flaviviruses such as Zika virus (ZIKV) induce reorganization of ER membranes to facilitate viral replication. Here, using 3D super resolution microscopy, ZIKV infection is shown to induce the formation of dense tubular matrices associated with viral replication in the central ER. Viral non-structural proteins NS4B and NS2B associate with replication complexes within the ZIKV-induced tubular matrix and exhibit distinct ER distributions outside this central ER region. Deep neural networks trained to distinguish ZIKV-infected versus mock-infected cells successfully identified ZIKV-induced central ER tubular matrices as a determinant of viral infection. Super resolution microscopy and deep learning are therefore able to identify and localize morphological features of the ER and allow for better understanding of how ER morphology changes due to viral infection.
Funder
CIHR Frederick Banting and Charles Best Canada Graduate Scholarship-Master’s award (CGS M).
Natural Sciences and Engineering Research Council of Canada
Canadian Institutes of Health Research
Canadian Institutes for Health Research
Publisher
Springer Science and Business Media LLC
Cited by
17 articles.
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