Author:
Almeida Sónia T.,Pedro Tânia,Paulo A. Cristina,de Lencastre Hermínia,Sá-Leão Raquel
Abstract
AbstractStreptococcus pneumoniae (pneumococcus) is a leading cause of infections worldwide. Disease is preceded by asymptomatic colonization of the upper respiratory tract. Classical culture-based methods (CCBM) suggest that colonization in the elderly is <5%. Recently, use of qPCR has challenged these observations. We estimated pneumococcal carriage prevalence and serotypes among Portuguese elderly using qPCR and compared results with those obtained by CCBM. Nasopharyngeal and oropharyngeal paired samples (599 each) of individuals over 60 years living in nursing (n = 299) or family (n = 300) homes were screened for the presence of pneumococci by qPCR targeting lytA and piaB. Positive samples were molecular serotyped. Use of qPCR improved detection of pneumococci in oropharyngeal samples compared to CCBM: from 0.7% to 10.4% (p < 0.001) in the nursing home collection, and from 0.3% to 5.0% (p < 0.001) in the family home collection. No significant differences were observed between both methods in nasopharyngeal samples (5.4% vs. 5.4% in the nursing homes; and 4.3% vs. 4.7% in the family homes). Twenty-one serotypes/serogroups were detected by qPCR compared to 14 by CCBM. In conclusion, use of qPCR suggests that pneumococcal carriage in Portuguese elderly is approximately 10%, and unveiled a large pool of serotypes. These results are important to understand progression to disease and impact of pneumococcal vaccines in the elderly.
Publisher
Springer Science and Business Media LLC
Reference41 articles.
1. CDC. Prevention of pneumococcal disease: recommendations of the advisory committee on the immunization practices (ACIP). Morbidity and Mortality Weekly Report (MMWR) 46, 1–24 (1997).
2. Ludwig, E., Bonanni, P., Rohde, G., Sayiner, A. & Torres, A. The remaining challenges of pneumococcal disease in adults. Eur Respir Rev 21, 57–65 (2012).
3. WHO. The top 10 causes of death. Geneva: World Health Organization, http://www.who.int/mediacentre/factsheets/fs310/en/index2.html [updated in June 2016]. Accessed on 21 July, 2019] (2013).
4. Converso, T. R., Assoni, L., André, G. O., Darrieux, M. & Leite, L. C. C. The long search for a serotype independent pneumococcal vaccine. Expert Rev Vaccines, https://doi.org/10.1080/14760584.2020.1711055 (2020).
5. Park, I. H. et al. Genetic, biochemical, and serological characterization of a new pneumococcal serotype, 6H, and generation of a pneumococcal strain producing three different capsular repeat units. Clin Vaccine Immunol 22, 313–318, https://doi.org/10.1128/CVI.00647-14 (2015).
Cited by
16 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献