Author:
Liu Huanhuan,Yang Lichun,Tu Zhonghua,Zhu Shenghua,Zhang Chengge,Li Huogen
Abstract
AbstractThe organogenesis and development of reproductive organs, i.e., stamen and gynoecium, are important floral characteristics that are closely related to pollinators and reproductive fitness. As a genus from Magnoliaceae,Liriodendronhas only two relict species:L. chinenseandL. tulipifera. Despite the similar flower shapes of these species, their natural seed-setting rates differ significantly, implying interspecies difference in floral organogenesis and development. MADS-box genes, which participate in floral organogenesis and development, remain unexplored inLiriodendron. Here, to explore the interspecies difference in floral organogenesis and development and identify MADS-box genes inLiriodendron, we examined the stamen and gynoecium primordia of the twoLiriodendronspecies by scanning electron microscopy combined with paraffin sectioning, and then collected two types of primordia for RNA-seq. A total of 12 libraries were constructed and 42,268 genes were identified, including 35,269 reference genes and 6,999 new genes. Monoterpenoid biosynthesis was enriched inL. tulipifera. Genome-wide analysis of 32 MADS-box genes was conducted, including phylogenetic trees, exon/intron structures, and conserved motif distributions. Twenty-six genes were anchored on 17 scaffolds, and six new genes had no location information. The expression profiles of MIKC-type genes via RT-qPCR acrossing six stamen and gynoecium developmental stages indicates that thePI-like,AG/STK-like,SEP-like, andSVP-likegenes may contribute to the species-specific differentiation of the organogenesis and development of reproductive organs inLiriodendron. Our findings laid the groundwork for the future exploration of the mechanism underlying on the interspecific differences in reproductive organ development and fitness inLiriodendron.
Funder
National Natural Science Foundation of China
Priority Academic Program Development of Jiangsu Higher Education Institutions
Publisher
Springer Science and Business Media LLC
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