Author:
Hambarde Shashank,Manalo Jeanne M.,Baskin David S.,Sharpe Martyn A.,Helekar Santosh A.
Abstract
AbstractRaising reactive oxygen species (ROS) levels in cancer cells to cause macromolecular damage and cell death is a promising anticancer treatment strategy. Observations that electromagnetic fields (EMF) elevate intracellular ROS and cause cancer cell death, have led us to develop a new portable wearable EMF device that generates spinning oscillating magnetic fields (sOMF) to selectively kill cancer cells while sparing normal cells in vitro and to shrink GBM tumors in vivo through a novel mechanism. Here, we characterized the precise configurations and timings of sOMF stimulation that produce cytotoxicity due to a critical rise in superoxide in two types of human glioma cells. We also found that the antioxidant Trolox reverses the cytotoxic effect of sOMF on glioma cells indicating that ROS play a causal role in producing the effect. Our findings clarify the link between the physics of magnetic stimulation and its mechanism of anticancer action, facilitating the development of a potential new safe noninvasive device-based treatment for GBM and other gliomas.
Funder
Kenneth R. Peak Foundation
John S. Dunn Foundation
Taub Foundation
Blanche Green Fund of the Pauline Sterne Wolff Memorial Foundation
Kelly Kicking Cancer Foundation
Methodist Hospital Foundation
Veralan Foundation
Many contributions in honor of Will McKone
Translational Research Initiative of Houston Methodist Research Institute
Publisher
Springer Science and Business Media LLC