Author:
Lichtenegger Antonia,Tamaoki Junya,Licandro Roxane,Mori Tomoko,Mukherjee Pradipta,Bian Lixuan,Greutter Lisa,Makita Shuichi,Wöhrer Adelheid,Matsusaka Satoshi,Kobayashi Makoto,Baumann Bernhard,Yasuno Yoshiaki
Abstract
AbstractBreast cancer is a leading cause of death in female patients worldwide. Further research is needed to get a deeper insight into the mechanisms involved in the development of this devastating disease and to find new therapy strategies. The zebrafish is an established animal model, especially in the field of oncology, which has shown to be a promising candidate for pre-clinical research and precision-based medicine. To investigate cancer growth in vivo in zebrafish, one approach is to explore xenograft tumor models. In this article, we present the investigation of a juvenile xenograft zebrafish model using a Jones matrix optical coherence tomography (JM-OCT) prototype. Immunosuppressed wild-type fish at 1-month post-fertilization were injected with human breast cancer cells and control animals with phosphate buffered saline in the tail musculature. In a longitudinal study, the scatter, polarization, and vasculature changes over time were investigated and quantified in control versus tumor injected animals. A significant decrease in birefringence and an increase in scattering signal was detected in tumor injected zebrafish in comparison to the control once. This work shows the potential of JM-OCT as a non-invasive, label-free, three-dimensional, high-resolution, and tissue-specific imaging tool in pre-clinical cancer research based on juvenile zebrafish models.
Funder
Austrian Science Fund
NIH/NICHD
American SIDS Institute
Vienna Science and Technology Fund
Japan Society for the Promotion of Science
Japan Science and Technology Agency
European Research Council
Publisher
Springer Science and Business Media LLC
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