Abstract
AbstractFowl cholera is a serious, highly contagious disease caused by the bacterium Pasteurella multocida (P. multocida) in a range of avian species and is characterized by an acute form of septicaemia. The pathogenic mechanism of chicken lung injury caused by the bacterium is unclear. Therefore, P. multocida Q (a reference standard strain isolated from chicken) and 1G1 (a clinic isolated strain from duck) were selected to infect chickens, establishing fowl cholera-induced laying hen models. Several important proteins involved in the process of lung injury were identified and quantified using immunohistochemistry and WB. The results showed that chicken lungs infected with bacteria for 24 h showed congestion and edema. The inflammatory factors HMGB1 and IL-6, intercellular matrix MMP, the cell apoptosis-associated caspase-3 and necrotic apoptosis signal molecules RIPK1 and RIPK3 were widely expressed in the lungs of group Q and were significantly different compared with those of 1G1 group and uninfected group (P < 0.05). The results indicated that RIPK1 and RIPK3 are involved in the injury process of chicken lungs after infection with P. multocida, and the mechanisms of lung injury induced by different strains are different.
Publisher
Springer Science and Business Media LLC
Reference43 articles.
1. Shi, S. H. et al. Development of PCR assay for detecting avian Pasteurella multocida. Fujian J. Agric. Sci. 24, 201–204 (2009).
2. Buttenschøn, J. Statistical evidence for a link between bronchopneumonia and disseminated focal nephritis in pigs. Zentralbl. Veterinarmed. A 38, 287–299 (1991).
3. Mackie, J. T., Barton, M. & Kettlewell, J. Pasteurella multocida septicaemia in pigs. Aust. Vet. J. 69, 227–228 (1992).
4. Ono, M. et al. Septicaemia and arthritis in pigs experimentally infected with Pasteurella multocida capsular serotype A. J. Comp. Pathol. 129, 251–258 (2003).
5. Pijoan, C. & Fuentes, M. Severe pleuritis associated with certain strains of Pasteurella multocida in swine. J. Am. Vet. Med. Assoc. 191, 823–826 (1987).
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献