Author:
Mylemans Bram,Lee Xiao Yin,Laier Ina,Helsen Christine,Voet Arnout R. D.
Abstract
Abstract$$\beta $$
β
-Propeller proteins are common natural disc-like pseudo-symmetric proteins that contain multiple repeats (‘blades’) each consisting of a 4-stranded anti-parallel $$\beta $$
β
-sheet. So far, 4- to 12-bladed $$\beta $$
β
-propellers have been discovered in nature showing large functional and sequential variation. Using computational design approaches, we created perfectly symmetric $$\beta $$
β
-propellers out of natural pseudo-symmetric templates. These proteins are useful tools to study protein evolution of this very diverse fold. While the 7-bladed architecture is the most common, no symmetric 7-bladed monomer has been created and characterized so far. Here we describe such a engineered protein, based on a highly symmetric natural template, and test the effects of circular permutation on its stability. Geometrical analysis of this protein and other artificial symmetrical proteins reveals no systematic constraint that could be used to help in engineering of this fold, and suggests sequence constraints unique to each $$\beta $$
β
-propeller sub-family.
Funder
Fonds Wetenschappelijk Onderzoek
KU Leuven
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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