Author:
Reis Laise Rodrigues,Costa-Rocha Ismael Artur,Abdala-Torres Thais,Campi-Azevedo Ana Carolina,Peruhype-Magalhães Vanessa,Araújo Márcio Sobreira Silva,Spezialli Elaine,do Valle Antonelli Lis Ribeiro,da Silva-Pereira Rosiane Aparecida,Almeida Gregório Guilherme,Fernandes Eder Gatti,Fantinato Francieli Fontana Sutile Tardetti,Domingues Carla Magda Allan Santos,Lemos Maria Cristina Ferreira,Chieppe Alexandre,Lemos Jandira Aparecida Campos,Coelho-dos-Reis Jordana Grazziela,de Lima Sheila Maria Barbosa,de Souza Azevedo Adriana,Schwarcz Waleska Dias,Camacho Luiz Antônio Bastos,de Lourdes de Sousa Maia Maria,de Noronha Tatiana Guimarães,Duault Caroline,Rosenberg-Hasson Yael,Teixeira-Carvalho Andréa,Maecker Holden Terry,Martins-Filho Olindo Assis, ,Otta Dayane Andriotti,Martins-Filho Olindo Assis
Abstract
AbstractThe present study aimed at evaluating the YF-specific neutralizing antibody profile besides a multiparametric analysis of phenotypic/functional features of cell-mediated response elicited by the 1/5 fractional dose of 17DD-YF vaccine, administered as a single subcutaneous injection. The immunological parameters of each volunteer was monitored at two time points, referred as: before (Day 0) [Non-Vaccinated, NV(D0)] and after vaccination (Day 30–45) [Primary Vaccinees, PV(D30–45)]. Data demonstrated high levels of neutralizing antibodies for PV(D30–45) leading to a seropositivity rate of 93%. A broad increase of systemic soluble mediators with a mixed profile was also observed for PV(D30–45), with IFN-γ and TNF-α presenting the highest baseline fold changes. Integrative network mapping of soluble mediators showed increased correlation numbers in PV(D30–45) as compared to NV(D0) (532vs398). Moreover, PV(D30–45) exhibited increased levels of Terminal Effector (CD45RA+CCR7−) CD4+ and CD8+ T-cells and Non-Classical memory B-cells (IgD+CD27+). Dimensionality reduction of Mass Cytometry data further support these findings. A polyfunctional cytokine profile (TNF-α/IFN-γ/IL-10/IL-17/IL-2) of T and B-cells was observed upon in vitro antigen recall. Mapping and kinetics timeline of soluble mediator signatures for PV(D30–45) further confirmed the polyfunctional profile upon long-term in vitro culture, mediated by increased levels of IFN-γ and TNF-α along with decreased production of IL-10. These findings suggest novel insights of correlates of protection elicited by the 1/5 fractional dose of 17DD-YF vaccine.
Publisher
Springer Science and Business Media LLC