Author:
Shimizu Yurika,Bandaru Srinivas,Hara Mari,Young Sonny,Sano Toshikazu,Usami Kaya,Kurano Yuta,Lee Suni,Kumagai-Takei Naoko,Takashiba Shogo,Sano Shunji,Ito Tatsuo
Abstract
AbstractWe herein elucidate the function of SARS-CoV-2derived 5'UTR in the human cells. 5'UTR bound host cellular RNAs were immunoprecipitated by gRNA-dCas13 (targeting luciferase RNA fused to SARS-CoV-2 5'UTR) in HEK293T and A549 cells. The 5'UTR bound RNA extractions were predominantly enriched for regulating lipid metabolism. Overexpression of SARS-CoV-2 5'UTR RNA altered the expression of factors involved in the process of the human Mevalonate pathway. In addition, we found that HMG-CoA reductase inhibitors were shown to suppress SARS-CoV-2 5'UTR-mediated translation activities. In conclusion, we deduce the array of host RNAs interacting with SARS-CoV-2 5'UTR that drives SARS-CoV-2 translation and influences host metabolic pathways.
Funder
KAKENHI Grant-in-Aid for Young Scientists
Kakenhi Grant Kiban C
Publisher
Springer Science and Business Media LLC
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