Author:
Kwok Andy T.,Mohamed Nequesha S.,Plate Johannes F.,Yammani Raghunatha R.,Rosas Samuel,Bateman Ted A.,Livingston Eric,Moore Joseph E.,Kerr Bethany A.,Lee Jingyun,Furdui Cristina M.,Tan Li,Bouxsein Mary L.,Ferguson Virginia L.,Stodieck Louis S.,Zawieja David C.,Delp Michael D.,Mao Xiao W.,Willey Jeffrey S.
Abstract
AbstractReduced knee weight-bearing from prescription or sedentary lifestyles are associated with cartilage degradation; effects on the meniscus are unclear. Rodents exposed to spaceflight or hind limb unloading (HLU) represent unique opportunities to evaluate this question. This study evaluated arthritic changes in the medial knee compartment that bears the highest loads across the knee after actual and simulated spaceflight, and recovery with subsequent full weight-bearing. Cartilage and meniscal degradation in mice were measured via microCT, histology, and proteomics and/or biochemically after: (1) ~ 35 days on the International Space Station (ISS); (2) 13-days aboard the Space Shuttle Atlantis; or (3) 30 days of HLU, followed by a 49-day weight-bearing readaptation with/without exercise. Cartilage degradation post-ISS and HLU occurred at similar spatial locations, the tibial-femoral cartilage-cartilage contact point, with meniscal volume decline. Cartilage and meniscal glycosaminoglycan content were decreased in unloaded mice, with elevated catabolic enzymes (e.g., matrix metalloproteinases), and elevated oxidative stress and catabolic molecular pathway responses in menisci. After the 13-day Shuttle flight, meniscal degradation was observed. During readaptation, recovery of cartilage volume and thickness occurred with exercise. Reduced weight-bearing from either spaceflight or HLU induced an arthritic phenotype in cartilage and menisci, and exercise promoted recovery.
Funder
National Institutes of Health
National Aeronautics and Space Administration
Publisher
Springer Science and Business Media LLC
Cited by
14 articles.
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