Author:
Bonassi Stefano,Ceppi Marcello,Møller Peter,Azqueta Amaya,Milić Mirta,Neri Monica,Brunborg Gunnar,Godschalk Roger,Koppen Gudrun,Langie Sabine A. S.,Teixeira João Paulo,Bruzzone Marco,Da Silva Juliana,Benedetti Danieli,Cavallo Delia,Ursini Cinzia Lucia,Giovannelli Lisa,Moretti Silvia,Riso Patrizia,Del Bo’ Cristian,Russo Patrizia,Dobrzyńska Malgorzata,Goroshinskaya Irina A.,Surikova Ekaterina I.,Staruchova Marta,Barančokova Magdalena,Volkovova Katarina,Kažimirova Alena,Smolkova Bozena,Laffon Blanca,Valdiglesias Vanessa,Pastor Susana,Marcos Ricard,Hernández Alba,Gajski Goran,Spremo-Potparević Biljana,Živković Lada,Boutet-Robinet Elisa,Perdry Hervé,Lebailly Pierre,Perez Carlos L.,Basaran Nursen,Nemeth Zsuzsanna,Safar Anna,Dusinska Maria,Collins Andrew,Anderson Diana,Andrade Vanessa,Pereira Cristiana Costa,Costa Solange,Gutzkow Kristine B.,Ladeira Carina,Moretti Massimo,Costa Carla,Orlow Irene,Rojas Emilio,Pourrut Bertrand,Kruszewski Marcin,Knasmueller Siegfried,Shaposhnikov Sergey,Žegura Bojana,Stopper Helga,
Abstract
AbstractThe comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
Funder
Italian Ministry of Health
European Cooperation in Science and Technology
Publisher
Springer Science and Business Media LLC