Author:
Shin Jeongwon,Kim Geon,Park Jinho,Lee Moosung,Park YongKeun
Abstract
AbstractThree-dimensional (3D) quantitative phase imaging (QPI) enables long-term label-free tomographic imaging and quantitative analysis of live individual bacteria. However, the Brownian motion or motility of bacteria in a liquid medium produces motion artifacts during 3D measurements and hinders precise cell imaging and analysis. Meanwhile, existing cell immobilization methods produce noisy backgrounds and even alter cellular physiology. Here, we introduce a protocol that utilizes hydrogels for high-quality 3D QPI of live bacteria maintaining bacterial physiology. We demonstrate long-term high-resolution quantitative imaging and analysis of individual bacteria, including measuring the biophysical parameters of bacteria and responses to antibiotic treatments.
Funder
Ministry of Science and ICT, South Korea
National Research Foundation of Korea
BK21+ program
KAIST UP program
Tomocube Inc.
Korea government
Publisher
Springer Science and Business Media LLC
Cited by
3 articles.
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