Purification of replicating pancreatic β-cells for gene expression studies

Author:

Carballar Reyes,Canyelles Maria de Lluc,Fernández Claudia,Martí YasminaORCID,Bonnin SarahORCID,Castaño Esther,Montanya Eduard,Téllez NoèliaORCID

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Reference37 articles.

1. Meier, J. J. et al. Beta-cell replication is the primary mechanism subserving the postnatal expansion of beta-cell mass in humans. Diabetes 57, 1584–1594 (2008).

2. Perl, S. et al. Significant human beta-cell turnover is limited to the first three decades of life as determined by in vivo thymidine analog incorporation and radiocarbon dating. J.Clin.Endocrinol.Metab 95, E234–E239 (2010).

3. Finegood, D., Scaglia, L. & Bonner-Weir, S. Dynamics of beta-cell mass in the growing rat pancreas. Estimation with a simple mathematical model. Diabetes 44, 249–256 (1995).

4. Montanya, E., Nacher, V., Biarnes, M. & Soler, J. Linear correlation between beta-cell mass and body weight throughout the lifespan in Lewis rats: role of beta-cell hyperplasia and hypertrophy. Diabetes 49, 1341–1346 (2000).

5. Tellez, N., Vilaseca, M., Martí, Y., Pla, A. & Montanya, E. β-cell dedifferentiation, reduced duct cell plasticity and impaired β-cell mass regeneration in middle-aged rats. Am J Physiol Endocrinol Metab, ajpendo. 00502, 02015 (2016).

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