Abstract
AbstractTransmembrane flux of Cs+(a K+congener) was measured in human red blood cells (RBCs; erythrocytes) on the 10-s time scale. This is the first report on dissolution dynamic nuclear polarization (dDNP) nuclear magnetic resonance (NMR) spectroscopy with this nuclide in mammalian cells. Four technical developments regularized sample delivery and led to high quality NMR spectra. Cation-free media with the Piezo1 (mechanosensitive cation channel) activator yoda1 maximized the extent of membrane transport. First-order rate constants describing the fluxes were estimated using a combination of statistical methods inMathematica, including the Markov chain Monte Carlo (MCMC) algorithm. Fluxes were in the range 4–70 μmol Cs+(L RBC)−1 s−1; these are smaller than for urea, but comparable to glucose. Methodology and analytical procedures developed will be applicable to transmembrane cation transport studies in the presence of additional Piezo1 effectors, to other cellular systems, and potentiallyin vivo.
Funder
Lundbeckfonden
Department of Education and Training | Australian Research Council
Publisher
Springer Science and Business Media LLC
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