Large-volume and deep brain imaging in rabbits and monkeys using COMPACT two-photon microscopy-Reference-Cited by-同舟云学术

Large-volume and deep brain imaging in rabbits and monkeys using COMPACT two-photon microscopy

Published:2022-10-22 Issue:1 Volume:12 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Lu Yuqing,Wei Xiangzan,Li Wei,Wu Xujun,Chen Chao,Li Ge,Huang Zhongqiang,Li Yunfeng,Zhang Yu,Gan Wen-Biao

Abstract

AbstractIn vivo imaging has been widely used for investigating the structure and function of neurons typically located within ~ 800 μm below the cortical surface. Due to light scattering and absorption, it has been difficult to perform in-vivo imaging of neurons in deep cortical and subcortical regions of large animals with two-photon microscopy. Here, we combined a thin-wall quartz capillary with a GRIN lens attached to a prism for large-volume structural and calcium imaging of neurons located 2 mm below the surface of rabbit and monkey brains. The field of view was greatly expanded by rotating and changing the depth of the imaging probe inside a quartz capillary. Calcium imaging of layer 5/6 neurons in the rabbit motor cortex revealed differential activity of these neurons between quiet wakefulness and slow wave sleep. The method described here provides an important tool for studying the structure and function of neurons located deep in the brains of large animals.

Funder

Shenzhen Key Science and Technology Infrastructure Planning Project

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

https://www.nature.com/articles/s41598-022-20842-z.pdf

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