Analysis of the difference between early-bolting and non-bolting roots of Angelica dahurica based on transcriptome sequencing
-
Published:2023-05-15
Issue:1
Volume:13
Page:
-
ISSN:2045-2322
-
Container-title:Scientific Reports
-
language:en
-
Short-container-title:Sci Rep
Author:
Wu Ping,Wang Xiaoyu,Guo Junxia,Zhang Songli,Li Qingmiao,Zhang Mei,Fang Qingmao,Luo Bin,Wang Hongsu,He Weijin
Abstract
AbstractAngelica dahurica (Fisch. ex Hoffm.) Benth.et Hook.f.var.formosana (Boiss.) Shan et Yuan (A. dahurica) is a well-known medicinal plant that has a wide range of applications in the pharmaceutical, food, cosmetic, and other industries. However, the issue of early bolting has emerged as a major hindrance to its production. This problem not only reduces the yield of A. dahurica, but also has an impact on its active ingredients. To date, the molecular factors that contribute to early bolting and its impact on the growth of A. dahurica have not been thoroughly investigated. Therefore, we conducted a transcriptome study using the Illumina NovaSeq 6000 on two developmental types: early-bolting and non-bolting (normal) roots of A. dahurica. We obtained 2,185 up-regulated and 1,414 down-regulated genes in total. Many of the identified transcripts were related to genes involved in early bolting. The gene ontology analysis revealed several differentially expressed genes that are crucial in various pathways, primarily associated with cellular, molecular, and biological processes. Additionally, the morphological characteristics and coumarin content in the early bolting roots of A. dahurica were significantly altered. This study provides insight into the transcriptomic regulation of early bolting in A. dahurica, which can potentially be utilized to enhance its medicinal properties.
Funder
Sichuan Basic Scientific Research Foundation
Sichuan Science and Technology Department Foundation
Chongqing Science and Technology Department Foundation
Sichuan Provincial Administration of Traditional Chinese Medicine Foundation
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Reference61 articles.
1. Kang, O. H. et al. Ethyl acetate extract from Angelica Dahuricae Radix inhibits lipopolysaccharide-induced production of nitric oxide, prostaglandin E2 and tumor necrosis factor-α via mitogen-activated protein kinases and nuclear factor-κB in macrophages. Pharmacol. Res. 55, 263–270 (2007).
2. Ji, Q., Ma, Y. H. & Zhang, Y. Research progress on chemical constituents and pharmacological effects of Angelicae Dahuricae Radix. Food Drug 22, 509–514 (2022).
3. Zhou, Y. & Na, L. X. Research progresson of medicinal and edible plant Angelica Dahurica. Asia-Pacific Tradit. Med. 18, 213–217 (2022).
4. Zhao, D. Y. et al. Advance in studying early bolting of Umbelliferae medicinal plant. China J. Chin. Mater.a Med. 41, 20–23 (2016).
5. Wang, M. Y. et al. Determination of coumarins content in radix Angelicae Dahuricae by HPLC and UV. J. Chin. Med. Mater. 27, 826–828 (2004).