Author:
Siao Ru-Fang,Lin Chia-Hsuan,Chen Li-Hsuan,Wang Liang-Chun
Abstract
AbstractTeleost fish skin serves as the first line of defense against pathogens. The interaction between pathogen and host skin determines the infection outcome. However, the mechanism(s) that modulate infection remain largely unknown. A proper tissue culture model that is easier to handle but can quantitatively and qualitatively monitor infection progress may shed some lights. Here, we use striped catfish (Pangasius hypophthalmus) to establish an ex vivo skin explant tissue culture model to explore host pathogen interactions. The skin explant model resembles in vivo skin in tissue morphology, integrity, and immune functionality. Inoculation of aquatic pathogen Aeromonas hydrophila in this model induces epidermal exfoliation along with epithelial cell dissociation and inflammation. We conclude that this ex vivo skin explant model could serve as a teleost skin infection model for monitoring pathogenesis under various infection conditions. The model can also potentially be translated into a platform to study prevention and treatment of aquatic infection on the skin in aquaculture applications.
Publisher
Springer Science and Business Media LLC
Reference57 articles.
1. Esteban, M. Á. An overview of the immunological defenses in fish skin. ISRN Immunol. 2012, 853470. https://doi.org/10.5402/2012/853470 (2012).
2. Elliott, D. G. Functional Morphology of the Integumentary System in Fishes. In: (ed. Farrell A.P.) Encyclopedia of Fish Physiology: From Genome to Environment, Vol. 1, 476–488. (San Diego, Academic Press 2011).
3. Rakers, S. et al. “Fish matters”: The relevance of fish skin biology to investigative dermatology. Exp. Dermatol. 19, 313–324. https://doi.org/10.1111/j.1600-0625.2009.01059.x (2010).
4. Dash, S., Das, S. K., Samal, J. & Thatoi, H. N. Epidermal mucus, a major determinant in fish health: A review. Iran. J. Vet. Res. 19, 72–81 (2018).
5. Lazado, C. C. & Caipang, C. M. Mucosal immunity and probiotics in fish. Fish Shellfish Immunol. 39, 78–89. https://doi.org/10.1016/j.fsi.2014.04.015 (2014).
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