Complementing two-photon fluorescence detection with backscatter detection to decipher multiparticle dynamics inside a nonlinear laser trap

Author:

Devi Anita,Yadav Sumit,De Arijit K.

Abstract

AbstractUsing wide-field and point detection modalities, we show how optical trapping dynamics under femtosecond pulsed excitation can be explored by complementing detection of two-photon fluorescence with backscatter. Radial trajectories of trapped particles are mapped from correlated/anti-correlated fluctuations in backscatter pattern whereas temporal evolution of two-photon fluorescence is used to mark the onset of trapping involving multiple particles. Simultaneous confocal detection of backscatter and two-photon fluorescence estimates axial trap stiffness, delineating short-time trapping dynamics. When a second particle is being trapped an oscillatory signal is observed which is due to interference of backscatter amplitudes, revealing inter-particle interactions within the trap. These findings are crucial steps forward to achieve controlled manipulation by harnessing optical nonlinearity under femtosecond pulsed excitation.

Funder

Indian Institute of Science Education and Research Mohali

University Grants Commission

Science and Engineering Research Board

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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