Author:
Aguilar-Vazquez Andrea,Chavarria-Avila Efrain,Salazar-Paramo Mario,Armendariz-Borunda Juan,Toriz-González Guillermo,Rodríguez-Baeza Marcela,Sandoval-Rodriguez Ana,Villanueva-Pérez Arisbeth,Godínez-Rubí Marisol,Medina-Preciado Jose-David,Lundberg Ingrid,Lozano-Torres Yesenia,Gomez-Rios Cynthia-Alejandra,Pizano-Martinez Oscar,Martinez-Garcia Erika-Aurora,Martin-Marquez Beatriz-Teresita,Duran-Barragan Sergio,Palacios-Zárate Brenda-Lucia,Llamas-Garcia Arcelia,Gómez-Limón Livier,Vazquez-Del Mercado Monica
Abstract
AbstractThe muscle fiber ultrastructure in Idiopathic Inflammatory Myopathies (IIM) has been scarcely explored, especially in Inclusion Body Myositis. The aim of this study was to implement the Scanning Electron Microscopy (SEM) in a small cohort of IIM patients, together with the characterization of immunological profile for a better understanding of the pathophysiology. For immunological profile characterization, we identified the presence of autoantibodies (Ro-52, OJ, EJ, PL7, PL12, SRP, Jo-1, PMScl75, PMScl100, Ku, SAE1, NXP2, MDA5, TIF1γ, Mi-2α, Mi-2β) and quantified cytokines (IL-1β, IFN-α2, IFN-γ, TNF-α, IL-6, IL-10, IL-12p70, IL-17A, IL-18, IL-23, IL-33) and chemokines (CCL2, CXCL8). The histological analysis was made by hematoxylin–eosin staining while the muscle fiber ultrastructure was characterized by SEM. We observed changes in the morphology and structure of the muscle fiber according to muscle strength and muscle enzymes. We were able to find and describe muscle fiber ultrastructure with marked irregularities, porosities, disruption in the linearity and integrity of the fascicle, more evident in patients with increased serum levels of muscle enzymes and diminished muscle strength. Despite the scarce reports about the use of SEM as a tool in all clinical phenotypes of IIM, our work provides an excellent opportunity to discuss and reframe the clinical usefulness of SEM in the diagnostic approach of IIM.
Funder
Fondo de Desarrollo Cientifico (FODECIJAL) 2019 from Consejo Estatal de Ciencia y Tecnología de Jalisco
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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