Author:
Milarska Sylwia E.,Androsiuk Piotr,Paukszto Łukasz,Jastrzębski Jan P.,Maździarz Mateusz,Larson Keith W.,Giełwanowska Irena
Abstract
AbstractThe genusCerastiumincludes about 200 species that are mostly found in the temperate climates of the Northern Hemisphere. Here we report the complete chloroplast genomes ofCerastium alpinum,C.arcticumandC.nigrescens. The length of cp genomes ranged from 147,940 to 148,722 bp. Their quadripartite circular structure had the same gene organization and content, containing 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Repeat sequences varied from 16 to 23 per species, with palindromic repeats being the most frequent. The number of identified SSRs ranged from 20 to 23 per species and they were mainly composed of mononucleotide repeats containing A/T units. Based on Ka/Ks ratio values, most genes were subjected to purifying selection. The newly sequenced chloroplast genomes were characterized by a high frequency of RNA editing, including both C to U and U to C conversion. The phylogenetic relationships within the genusCerastiumand family Caryophyllaceae were reconstructed based on the sequences of 71 protein-coding genes. The topology of the phylogenetic tree was consistent with the systematic position of the studied species. All representatives of the genusCerastiumwere gathered in a single clade withC. glomeratumsharing the least similarity with the others.
Publisher
Springer Science and Business Media LLC
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