Author:
Li Hong-Hui,Bao Ling-Sheng,Deng Shi-Ming,Liu Li,Cheng Jia,Chen Xiao,Pan Ya-Xiong,Zhang Jian-She,Chu Wu-Ying
Abstract
AbstractMuscle is an important structural tissue in aquatic animals and it is susceptible to bacterial and fungal infection, which could affect flesh quality and health. In this study, Chinese soft-shelled turtles were artificially infected with two pathogens, Proteus vulgaris and Elizabethkingia meningoseptica and the effects on muscle nutritional characteristics, oxidative stress and autophagy were assayed. Upon infection, the muscle nutritional composition and muscle fiber structure were notably influenced. Meanwhile, the mRNA expression of Nrf2 was down-regulated and Keap1 up-regulated, thus resulting in a decrease in antioxidant capacity and oxidative stress. However, with N-acetylcysteine treatment, the level of oxidative stress was decreased, accompanied by significant increases in antioxidant enzyme activities and the mRNA levels of SOD, CAT, GSTCD, and GSTO1. Interestingly, there was a significant increase in autophagy in the muscle tissue after the pathogen infection, but this increase could be reduced by N-acetylcysteine treatment. Our findings suggest that muscle nutritional characteristics were dramatically changed after pathogen infection, and oxidative stress and autophagy were induced by pathogen infection. However, N-acetylcysteine treatment could compromise the process perhaps by decreasing the ROS level and regulating Nrf2-antioxidant signaling pathways.
Funder
Scientific Research Foundation of Hunan Provincial Education Department
Hunan Provincial Science and Technology Department
National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Reference62 articles.
1. Shi, J., Chu, W. Y. & Zhang, J. S. Muscle growth, differentiation and gene expression regulation in fish. Acta Hydrobiol. Sin. 37, 1145–1152 (2013).
2. Wernerman, J., Luo, J. & Hammarqvist, F. Glutathione status in critically-ill patients: possibility of modulation by antioxidants. Proc. Nutr. Soc. 58, 677–680 (1999).
3. Wu, C. L., Satomi, Y. & Walsh, K. RNA-seq and metabolomic analyses of Akt1-mediated muscle growth reveals regulation of regenerative pathways and changes in the muscle secretome. BMC Genom. 18, 181 (2017).
4. Zigangirova, N. A., Nesterenko, L. N., Tiganova, I. L. & Kost, E. A. The role of the type-III secretion system of Gram-negative bacteria in the regulation of chronic infections. Mol. Genet. Microbiol. 27, 91–102 (2012).
5. Rzigalinski, B. A., Hockey, K. S., Palmieri, J. R. & Elswaifi, S. F. Antioxidant nanoparticles for control of infectious disease. Infect. Disord. Drug Targets 9, 445–452 (2009).
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