Abstract
AbstractCassava root-rot incited by soil-borne pathogens is one of the major diseases that reduces root yield. Although the use of resistant cultivars is the most effective method of management, the genetic basis for root-rot resistance remains poorly understood. Therefore, our work analyzed the transcriptome of two contrasting genotypes (BRS Kiriris/resistant and BGM-1345/susceptible) using RNA-Seq to understand the molecular response and identify candidate genes for resistance. Cassava seedlings (resistant and susceptible to root-rot) were both planted in infested and sterilized soil and samples from Initial-time and Final-time periods, pooled. Two controls were used: (i) seedlings collected before planting in infested soil (absolute control) and, (ii) plants grown in sterilized soil (mock treatments). For the differentially expressed genes (DEGs) analysis 23.912 were expressed in the resistant genotype, where 10.307 were differentially expressed in the control treatment, 15 DEGs in the Initial Time-period and 366 DEGs in the Final Time-period. Eighteen candidate genes from the resistant genotype were related to plant defense, such as the MLP-like protein 31 and the peroxidase A2-like gene. This is the first model of resistance at the transcriptional level proposed for the cassava × root-rot pathosystem. Gene validation will contribute to screening for resistance of germplasm, segregating populations and/or use in gene editing in the pursuit to develop most promising cassava clones with resistance to root-rot.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa do Estado da Bahia
Foreign, Commonwealth and Development Office
Bill and Melinda Gates Foundation
Publisher
Springer Science and Business Media LLC
Reference116 articles.
1. Foundation Agricultural Organization (FAO). Save and Grow: Cassava. A Guide to Sustainable Production Intensification (FAO, 2013).
2. Foundation Agricultural Organization (FAO). Food Outlook: Biannual Report on Global Food Markets (FAO, 2018).
3. McCallum, E. J., Anjanappa, R. B. & Gruissem, W. Tackling agriculturally relevant diseases in the staple crop cassava (Manihot esculenta). Curr. Opin. Plant Biol. 38, 50–58 (2017).
4. Akrofi, S., Akuoko, K., Bour, K. & Nyarko, A. Farmers’ knowledge and perception of the dry cassava root rot disease in Brong Ahafo region of Ghana. Ghana J. Agric. Sci. 52, 33–42 (2018).
5. Boari, A. J., Cunha, E. M., Quadros, A. F. F., Barreto, R. W. & Fernandes, A. F. First report of Phytopythium sp. causing storage root rot and foliage blight of cassava in Brazil. Plant Dis. 102, 1042–1042 (2018).