Author:
Kim Hyung Il,Kim Jinhyeon,Kim Hyori,Lee Hyeri,Yoon Yong Sik,Hwang Sung Wook,Park Sang Hyoung,Yang Dong-Hoon,Ye Byong Duk,Byeon Jeong-Sik,Yang Suk-Kyun,Kim Sun Young,Myung Seung-Jae
Abstract
AbstractFor the sensitive diagnosis of colorectal cancer lesions, advanced molecular imaging techniques using cancer-specific targets have emerged. However, issues regarding the clearance of unbound probes and immunogenicity remain unresolved. To overcome these limitations, we developed a small-sized scFv antibody fragment conjugated with FITC for the real-time detection of colorectal cancer by in vivo molecular endoscopy imaging. A small-sized scFv fragment can target colon cancer secreted protein-2 (CCSP-2), highly expressed in colorectal adenocarcinoma tissues; moreover, its full-length IgG probe has been used for molecular imaging previously. To assess the efficacy of anti-CCSP-2 scFv-FITC, surgical specimens were obtained from 21 patients with colorectal cancer for ex vivo molecular fluorescence analysis, histology, and immunohistochemistry. Orthotopic mice were administered with anti-CCSP-2 scFv-FITC topically and intravenously, and distinct tumor lesions were observed by real-time fluorescence colonoscopy. The fluorescence imaging of human colon cancer specimens allowed the differentiation of malignant tissues from non-malignant tissues (p < 0.05), and the CCSP-2 expression level was found to be correlated with the fluorescence intensity. Here, we demonstrated the feasibility and safety of anti-CCSP-2 scFv-FITC for molecular imaging as well as its potential in real-time fluorescence colonoscopy for the differential diagnosis of tumor lesions.
Funder
National Research Foundation of Korea (NRF) funded by the Ministry of Education
Grant of Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea.
Korea Health Technology R&D Project through the Korea Health Industry Development Institute
Publisher
Springer Science and Business Media LLC
Cited by
3 articles.
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