Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/s41598-018-36691-8.pdf
Reference56 articles.
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3. Feige, M. J., Hendershot, L. M. & Buchner, J. How antibodies fold. Trends Biochem Sci. 35, 189–198 (2010).
4. Kawasaki, T., Gouda, M. D., Sawasaki, T., Takai, K. & Endo, Y. Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ. Eur J Biochem. 270, 4780–4786 (2003).
5. Merk, H., Gless, C., Maertens, B., Gerrits, M. & Stiege, W. Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomes. Biotechniques. 53, 153–160 (2012).
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