Author:
Zwanenburg Laurens,Borloo Jimmy,Decorte Bregt,Bunte Myrna J. M.,Mokhtari Sanaz,Serna Sonia,Reichardt Niels-C.,Seys Leen J. M.,van Diepen Angela,Schots Arjen,Wilbers Ruud H. P.,Hokke Cornelis H.,Claerebout Edwin,Geldhof Peter
Abstract
AbstractThe development of effective recombinant vaccines against parasitic nematodes has been challenging and so far mostly unsuccessful. This has also been the case for Ostertagia ostertagi, an economically important abomasal nematode in cattle, applying recombinant versions of the protective native activation-associated secreted proteins (ASP). To gain insight in key elements required to trigger a protective immune response, the protein structure and N-glycosylation of the native ASP and a non-protective Pichia pastoris recombinant ASP were compared. Both antigens had a highly comparable protein structure, but different N-glycan composition. After mimicking the native ASP N-glycosylation via the expression in Nicotiana benthamiana plants, immunisation of calves with these plant-produced recombinants resulted in a significant reduction of 39% in parasite egg output, comparable to the protective efficacy of the native antigen. This study provides a valuable workflow for the development of recombinant vaccines against other parasitic nematodes.
Funder
Fonds Wetenschappelijk Onderzoek
Horizon 2020 Framework Programme
International Coordination of Research on Infectious Animal Diseases 2021
Ministry of Science and Education (MINECO),Spain
Agencia Estatal de Investigación
Maria de Maeztu Units of Excellence Program
Basque Government
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Publisher
Springer Science and Business Media LLC